RELION

Following the RELION Tutorial Workflow (2D → 3D)

Following the official RELION tutorial workflow, this post covers Import, Motion Correction, CTF Estimation, Auto-picking, Extraction, 2D Classification, Initial Model, and 3D Refinement with all key GUI parameters.

Installing RELION and Running the Tutorial Dataset on Ubuntu

How to install RELION 5.0 on Ubuntu using the official relion-5.0 Conda environment, set up CUDA paths, and verify the installation with the RELION tutorial data.

Setting Up a Unified CryoSPARC / RELION Environment on Ubuntu

Step-by-step instructions for setting up CryoSPARC and RELION on Ubuntu 24.04 with CUDA configuration and external SSD-based data management.

Building a Workstation for Single-Particle Analysis (SPA)

Learn how to build two types of SPA workstations — one for full-scale Cryo-EM research and one for budget-friendly learning — using Intel Xeon and i9 processors.

Why a Workstation is Essential for Single-Particle Analysis (SPA)

A practical guide to building a training-grade workstation for SPA in Cryo-EM and Negative-stain EM.