Tutorial

Following the RELION Tutorial Workflow (2D → 3D)

Following the official RELION tutorial workflow, this post covers Import, Motion Correction, CTF Estimation, Auto-picking, Extraction, 2D Classification, Initial Model, and 3D Refinement with all key GUI parameters.

Installing RELION and Running the Tutorial Dataset on Ubuntu

How to install RELION 5.0 on Ubuntu using the official relion-5.0 Conda environment, set up CUDA paths, and verify the installation with the RELION tutorial data.